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Table 1 Fold expression changes of HSP, f.a., etc. related genes at 28 vs. 21 °C, based on RT-PCR and RNA-Seq analyses

From: The damage and tolerance mechanisms of Phaffia rhodozyma mutant strain MK19 grown at 28 °C

 

Gene

Function

RNA-Seq: fold-change 28/21 °C

RT-PCR:

fold-change 28/21 °C

1

comp12727_c0

HSP

1.75

1.93

2

comp13502_c0

HSP homolog pss1

16.16

3.87

3

comp12521_c0

DnaJ family

1.25

-1.29

4

comp11731_c2

HSP

2.25

8.65

5

comp11698_c0

Stress-induced protein STI1

2.63

2.33

6

comp12783_c0

HSP104

2.58

4.35

7

comp12308_c0

HSP90

12.06

3.60

8

comp11869_c0

HSP60

2.77

2.71

9

comp12396_c0

HSP78

2.97

4.50

10

comp10325_c0

HSP SSB

2.45

2.23

11

comp11696_c0

hsp70-like protein

3.74

3.65

12

comp12989_c0

HSP70

5.66

N.D.

13

comp13599_c0

FAS2

0.29

N.D.

14

comp13900_c0

FAS1

0.40

N.D.

15

comp13834_c0

ACC1, acetyl-CoA carboxylase

0.25

N.D.

16

comp11956_c0

f.a.-2 hydroxylase

0.48

N.D.

17

comp12194_c0

f.a. desaturase

0.32

N.D.

18

comp10129_c0

WSC domain-containing protein

0.03

N.D.

19

comp10153_c0

WSC

1.80

N.D.

20

comp10598_c1

WSC domain-containing protein

0.18

N.D.

21

comp10909_c0

Related to WSC2 glucoamylase III

0.77

N.D.

22

comp10944_c0

WSC domain-containing protein

0.03

N.D.

23

comp11101_c1

WSC

1.52

N.D.

24

comp11381_c0

WSC domain-containing protein

0.02

N.D.

25

comp11733_c0

WSC

0.30

N.D.

26

comp12382_c1

WSC-domain-containing protein

0.04

N.D.

27

comp12404_c0

WSC

0.15

N.D.

28

comp12789_c0

WSC

0.82

N.D.

29

comp12838_c0

WSC

0.11

N.D.

30

comp11864_c1

Alpha-1,3-mannosyltransferase CMT1

2.82

N.D.

31

comp12286_c0

Glycosyltransferase family 22 protein

3.88

N.D.

32

comp12082_c1

Alpha-1,6-mannosyltransferase

0.53

N.D.

33

comp11585_c0

Glycosyltransferase family 22 protein

1.95

N.D.

34

comp13450_c0

1,3-beta-glucanosyltransferase

0.45

N.D.

35

comp11848_c1

Endo-1,3(4)-beta-glucanase

0.27

N.D.

36

comp11848_c2

Endo-1,3(4)-beta-glucanase

0.31

N.D.

37

comp11853_c0

Endo-1,3(4)-beta-glucanase

2.89

N.D.

38

comp12961_c0

Glucan endo-1,3-alpha-glucosidase agn1

0.33

N.D.

39

comp10598_c0

Glycoside hydrolase family 71 protein

0.14

N.D.

40

comp13745_c1

Chitin deacetylase

4.50

N.D.

41

comp14086_c0

Glycosyltransferase family 2 protein

2.41

N.D.

42

comp12056_c0

Chitin synthase 1

1.75

N.D.

43

comp12623_c0

Glycosyltransferase family 2 protein

1.99

N.D.

44

comp13627_c0

Glycosyltransferase family 2 protein

2.2

N.D.

45

comp13980_c0

Chitin synthase 6

2.2

N.D.

46

comp11755_c0

HMG-CoA synthase A

0.80

1.39

47

comp13410_c0

HMG-CoA reductase

1.03

1.08

48

comp13164_c0

FPP synthase

1.12

1.50

49

comp10042_c0

IPP isomerase

0.87

2.78

  1. N.D. not detected
  2. No. 1–12: HSP genes. No. 13–17: f.a. biosynthetic pathway genes. No. 18–29: WSC genes. No. 30–33: mannan biosynthetic pathway genes. No. 34–39: glucan biosynthetic pathway genes. No. 40–45: chitin biosynthetic pathway genes.No. 46–49: MVA pathway genes
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Microbial Cell Factories

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