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Table 1 Fold expression changes of HSP, f.a., etc. related genes at 28 vs. 21 °C, based on RT-PCR and RNA-Seq analyses

From: The damage and tolerance mechanisms of Phaffia rhodozyma mutant strain MK19 grown at 28 °C

  Gene Function RNA-Seq: fold-change 28/21 °C RT-PCR:
fold-change 28/21 °C
1 comp12727_c0 HSP 1.75 1.93
2 comp13502_c0 HSP homolog pss1 16.16 3.87
3 comp12521_c0 DnaJ family 1.25 -1.29
4 comp11731_c2 HSP 2.25 8.65
5 comp11698_c0 Stress-induced protein STI1 2.63 2.33
6 comp12783_c0 HSP104 2.58 4.35
7 comp12308_c0 HSP90 12.06 3.60
8 comp11869_c0 HSP60 2.77 2.71
9 comp12396_c0 HSP78 2.97 4.50
10 comp10325_c0 HSP SSB 2.45 2.23
11 comp11696_c0 hsp70-like protein 3.74 3.65
12 comp12989_c0 HSP70 5.66 N.D.
13 comp13599_c0 FAS2 0.29 N.D.
14 comp13900_c0 FAS1 0.40 N.D.
15 comp13834_c0 ACC1, acetyl-CoA carboxylase 0.25 N.D.
16 comp11956_c0 f.a.-2 hydroxylase 0.48 N.D.
17 comp12194_c0 f.a. desaturase 0.32 N.D.
18 comp10129_c0 WSC domain-containing protein 0.03 N.D.
19 comp10153_c0 WSC 1.80 N.D.
20 comp10598_c1 WSC domain-containing protein 0.18 N.D.
21 comp10909_c0 Related to WSC2 glucoamylase III 0.77 N.D.
22 comp10944_c0 WSC domain-containing protein 0.03 N.D.
23 comp11101_c1 WSC 1.52 N.D.
24 comp11381_c0 WSC domain-containing protein 0.02 N.D.
25 comp11733_c0 WSC 0.30 N.D.
26 comp12382_c1 WSC-domain-containing protein 0.04 N.D.
27 comp12404_c0 WSC 0.15 N.D.
28 comp12789_c0 WSC 0.82 N.D.
29 comp12838_c0 WSC 0.11 N.D.
30 comp11864_c1 Alpha-1,3-mannosyltransferase CMT1 2.82 N.D.
31 comp12286_c0 Glycosyltransferase family 22 protein 3.88 N.D.
32 comp12082_c1 Alpha-1,6-mannosyltransferase 0.53 N.D.
33 comp11585_c0 Glycosyltransferase family 22 protein 1.95 N.D.
34 comp13450_c0 1,3-beta-glucanosyltransferase 0.45 N.D.
35 comp11848_c1 Endo-1,3(4)-beta-glucanase 0.27 N.D.
36 comp11848_c2 Endo-1,3(4)-beta-glucanase 0.31 N.D.
37 comp11853_c0 Endo-1,3(4)-beta-glucanase 2.89 N.D.
38 comp12961_c0 Glucan endo-1,3-alpha-glucosidase agn1 0.33 N.D.
39 comp10598_c0 Glycoside hydrolase family 71 protein 0.14 N.D.
40 comp13745_c1 Chitin deacetylase 4.50 N.D.
41 comp14086_c0 Glycosyltransferase family 2 protein 2.41 N.D.
42 comp12056_c0 Chitin synthase 1 1.75 N.D.
43 comp12623_c0 Glycosyltransferase family 2 protein 1.99 N.D.
44 comp13627_c0 Glycosyltransferase family 2 protein 2.2 N.D.
45 comp13980_c0 Chitin synthase 6 2.2 N.D.
46 comp11755_c0 HMG-CoA synthase A 0.80 1.39
47 comp13410_c0 HMG-CoA reductase 1.03 1.08
48 comp13164_c0 FPP synthase 1.12 1.50
49 comp10042_c0 IPP isomerase 0.87 2.78
  1. N.D. not detected
  2. No. 1–12: HSP genes. No. 13–17: f.a. biosynthetic pathway genes. No. 18–29: WSC genes. No. 30–33: mannan biosynthetic pathway genes. No. 34–39: glucan biosynthetic pathway genes. No. 40–45: chitin biosynthetic pathway genes.No. 46–49: MVA pathway genes
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Microbial Cell Factories

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