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Table 3 Primer sequences of RT-qPCR used for the analyses of inflammatory and anti-inflammatory gene expressions of LPS-stimulated THP-1 macrophage cells

From: Anti-cancer and anti-inflammatory effects elicited by short chain fatty acids produced by Escherichia coli isolated from healthy human gut microbiota

  Specific gene Primer
Cytokines gene IL-1β F-GTGGCAATGAGGATGACTTGTTC
R-TAGTGGTGGTCGGAGATTCGTA
IL-6 F-AGCCACTCACCTCTTCAGAAC
R-GCCTCTTTGCTGCTTTCACAC
IL-8 F-CTGATTTCTGCAGCTCTGTG
R-GGGTGGAAAGGTTTGGAGTATG
IL-10 F-GTGATGCCCCAAGCTGAGA
R-CACGGCCTTGCTCTTGTTTT
TNF-α F-CTGCTGCACT TTGGAGTGAT
R-AGATGATCTG ACTGCCTGGG
Housekeeping gene ACTB (β-actin) F-ATTGCCGACAGGATGCAGAA
R-GCTGATCCACATCTGCTGGAA
GAPDH
(Glyceraldehyde-3-phosphatedehydrogenase)
F-CAACAGCGACACCCACTCCT
R-CACCCTGTTGCTGTAGCCAAA