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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Metabolic engineering of Saccharomyces cerevisiae for high-level production of gastrodin from glucose

Fig. 2

The de no biosynthetic pathway of gastrodin and genes arrangement in rDNA locus or δ sites. Single arrows represent one-step conversion, double arrows represent multi-step conversion, single arrow with cross lines represents pathway block. Abbreviations: PEP, phosphoenolpyruvate; E4P, erythrose 4-phosphate; DAHP, 3-deoxy-arabino-heptulonate-7-phosphate; EPSA, 5-enolpyruvyl-shikimate-3-phosphate; PPA, prephenate; 4-HBA, 4-hydroxybenzoic acid; PpsA, phosphoenolpyruvate synthase; TktA, transketolase; ARO4K229L, feedback-resistant DAHP synthase mutant; ARO1, pentafunctional EPSP synthase; ARO2, chorismate synthase gene; UbiC, chorismate pyruvate-lyase; CAR, carboxylic acid reductase; PPTcg-1, phosphopantetheinyl transferase; ADHS, alcohol dehydrogenases; AsUGT, glucosyltransferase; ARO7, chorismate mutase. PTDH3, TDH3 promoter; PScTEF1, ScTEF1 promoter; PPGK1, PGK1 promoter. Genes with ‘syn’ represent codon-optimized genes in S. cerevisiae

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