Fig. 1

Chromatography to isolate an antifungal protein. Elution profile of the antifungal protein in the 80% ammonium sulphate precipitate separated using DEAE Sepharose Fast Flow ion-exchange chromatography (a). The antifungal activities of the separated proteins against the wheat pathogen Rhizoctonia cerealis (b). A–H represent separated fractions from the precipitate of the Z-14 strain fermentation supernatant [30 µg of each separated fraction dissolved in 30 µl of 10 mmol/l Tris–HCl buffer (pH 7.5)]; CK represents the negative control comprising 30 µl of 10 mmol/l Tris–HCl buffer (pH 7.5)