Fig. 4

CoA-dependent pathways to propane and butane via clostridial butanol pathway [9] and reverse β-oxidation [20, 59]. The homologous route is constructed with enzymes from Cupriavidus necator and Treponema denticola. Dashed arrows indicate proposed alternative routes not currently tested. adhE2: = bi-functional aldehyde-alcohol dehydrogenase. Other enzymes are defined in the Fig. 2 legend