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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Engineering nature for gaseous hydrocarbon production

Fig. 2

Biosynthetic pathways developed for the production of propane, butane and isobutane. Chemicals highlighted in red and green are bio-alkane gas precursors that could be fed into cultures for single step CvFAP-dependent and amino acid metabolism pathways, respectively. Enzymes: Acc: acetyl-CoA carboxylase; ADH: Aldehyde dehydrogenase homologue; AdhE2: bi-functional aldehyde-alcohol dehydrogenases; ADO/Ferr: aldehyde-deformylating oxygenase and electron transfer partner ferredoxin; alsS: acetolactate synthase; AtoB: acetyl-CoA acetyltransferase; bcd: butyryl-CoA dehydrogenase; BktB: β-ketothiolase; BCKDHAB: human 2-oxoisovalerate dehydrogenase; CAR/sfp: carboxylic acid reductase and activating enzyme 4′-phosphopantetheinyl transferase; Crt: 3-hydroxybutyryl-CoA dehydratase; CvFAP: fatty acid photodecarboxylase; FabA: β-hydroxyacyl-ACP dehydrase; FabDH: malonyl-CoA-ACP transacetylase and β-ketoacyl-ACP synthase; FabG: β-ketoacyl-ACP reductase; FabI: enoyl-ACP reductase; Hbd: 3-hydroxybutyryl-CoA dehydrogenase; ilvA: L-threonine dehydratase biosynthetic; ilvC: ketol-acid reductoisomerase; ilvD: dihydroxy-acid dehydratase; ilvE: branched-chain amino acid aminotransferase; KdcA: branched-chain keto acid decarboxylase; LeuABCD: isopropyl malate synthase, dehydrogenase & isomerases complex; NphT7: acetoacetyl CoA synthase; phaB: acetoacetyl-CoA reductase; phaJ4b: R-specific enoyl CoA hydratase 4b; PyDH: pyruvate dehydrogenase; Ter: trans-2-enoyl-CoA reductase; Tes4: acyl-ACP thioesterase; thl: thiolase; YciA: acyl-CoA thioester hydrolase

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