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Table 1 Growth and substrate consumption rates of cells during main growth phase at 95% (12–18 h), 1% (10–17 h) and 0% (17–23 h) dO2

From: An automated oxystat fermentation regime for microoxic cultivation of Magnetospirillum gryphiswaldense

  95% dO2 1% dO2 0% dO2
Growth rate µ [h−1] 0.07 ± 0.009 0.15 ± 0.007 0.13 ± 0.005
Doubling time [h] 9.61 ± 1.3 4.76 ± 0.23 5.24 ± 0.21
Maximal OD565 0.71 ± 0.05 1.06 ± 0.06 0.49 ± 0.01
Lactate consumption rate [mM h−1 OD −1565 ] 2.67 ± 1.17 4.63 ± 1.02 3.12 ± 1.17
Yx/s [mgdw mmol −1lactate ] 4.59 ± 0.88 4.08 ± 0.43 5.40 ± 1.04
Nitrate consumption rate [mM h−1 OD −1565 ] 0.71 ± 0.28 1.09 ± 0.15 6.13 ± 0.83
Yx/s [mgdw mmol −1nitrate ] 26.5 ± 4.6 21.1 ± 4.2 2.5 ± 0.9
Cellular iron content [mg g −1dw ] 3.3 ± 0.7 14.4 ± 0.4 25.3 ± 0.9
  1. Rates were measured in biological triplicates. Iron content of the biomass was measured after the end of cultivation at 35 h. Biomass productivity per mol substrate for lactate and nitrate (Yx/s) was calculated for the end of growth, where OD565 reached the maximum