Table 3 Oligonucleotides used in this study
From: Construction and description of a constitutive plipastatin mono-producing Bacillus subtilis
Name | Sequence 5′–3′ | Purpose |
|---|---|---|
s1009 | CTGCCGTTATTCGCTGGATT | Integration of degQ gene (B. subtilis DSM10T) (+ 510 bp) in amyE locus underlined sequences highlight the Nde I and EcoR I restriction site |
s1410 | ATTATTAACATATGCGGCGTACCTCATACGGATACAC | |
s1409 | ATTATTAAGAATTCCTCCTTGATCCGGACAGAATC | |
s1010 | AGAGAACCGCTTAAGCCCGA | |
s1221 | GGAAAGTGAAAAAAGGAGAAGG | Construction of Pveg::Ppps promoter exchange |
s1222 | CCTATGCAGGTTTTCAACTGTTATTGATTTGCCAAAATGACAG | |
s1223 | CAGTTGAAAACCTGCATAGG | |
s1224 | TGCATCCACCTCACTACAT | |
s1225 | ATGTAGTGAGGTGGATGCATTGAGCGAACATACTTATTCTTTAAC | |
s1226 | CATTTAAAGAGATTCCATCCATTATGATATG | |
s1162 | CATGATTTTCAGGTCTGCAAGAAC | Construction of srfAA-AD:: comS-erm |
s1163 | GTTCAAACGTCTGCTCCTCCTTAATCTTTATAAGCAGTGAACATGTGC | |
s1164 | AGGAGGAGCAGACGTTTGAAC | |
s1165 | CTTCTCCCTCCAGCAGAAGTAC | |
s1166 | CTTCTGCTGGAGGGAGAAGTAGGTATAAATTTAAC-GATCACTCATCATGTTC | |
s1167 | GACCGATAGATTTTGAATTTAGGTGTC | |
s1168 | CACCTAAATTCAAAATCTATCGGTCGAATGCCAAT-TTCTGCATGGTATAATAG | |
s1169 | GGCAACCTGATGGATAAAGAAATTG |