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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Construction and description of a constitutive plipastatin mono-producing Bacillus subtilis

Fig. 3

A Detected HPTLC chromatograms of the standard plipastatin in comparison with plipastatin produced by BMV9 (3NA sfp +) and BMV12 (3NA plipastatin mono-producer) with and without ornithine supplementation. Plipastatin standard produced by B. subtilis (Lipofabrik, France) [a], plipastatin produced by BMV9 strain after 48 h cultivation in mineral salt medium without any amino acid and with ornithine supplementation [b]; plipastatin produced by BMV12 strain after 48 h cultivation in mineral salt medium without amino acid and with ornithine supplementation [c]. B Comparison of plipastatin variants ratios in plipastatin standard and mono-producer BMV12 strain during exponential and stationary phase. The BMV12 was cultivated in mineral salt medium supplemented with 30 mM of ornithine. The samples were taken after 24 h (exponential phase) and 48 h (stationary phase). Peak areas were determined by extracted ion chromatograms for each plipastatin variant using their precise m/z values. Ratios of the peak areas of plipastatin variants were calculated within the standard and the two sample points

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