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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Efficient protection of microorganisms for delivery to the intestinal tract by cellulose sulphate encapsulation

Fig. 5

Survival of Encapsulated Bacteria and Yeast after acid exposure followed by bile. a After overnight culture of L. casei (b) or S. boulardii (c), or encapsulation of overnight pre-cultures, the free or CS encapsulated L. casei (b) or S. boulardii (c) were freeze dried and stored before rehydration and either direct titration or decapsulation followed by titration to determine the CFU per mL or per capsule. The CFU per 50 capsules was set as 1, and equivalent CFU of non-encapsulated bacteria or yeast also used in the “free, non-encapsulated” samples. 50 capsules or the equivalent CFU of non-encapsulated L. casei (b) or S. boulardii (c) was then subjected to exposure to AGJ + P (filled square—red lines, black up-pointing triangle—green lines), or to AGJ at pH 7 (filled diamond—blue lines, filled circle—orange lines) for up to 4 h, followed by exposure to artificial bile for 1 h. This was followed either by direct titration, or titration after decapsulation, on appropriate agar plates. The resulting measured CFU were expressed as relative viability compared to the initial CFU count (before acid or bile exposure) that was set as 1. b and c Time course of normalized survival of encapsulated (filled square—red lines) or free, non-encapsulated (black up-pointing triangle—green lines) L. casei (b), and S. boulardii (c after up to 4 h exposure to artificial gastric juice plus pepsin and lysozyme (AGJ + P) followed by one hour exposure to artificial bile. For comparison the time course of survival of encapsulated (black up-pointing triangle—blue lines) or free, non-encapsulated (filled circle—orange lines) L. casei (b), and S. boulardii (c) after 4 h exposure to artificial gastric juice at pH 7 (AGJ) followed by one hour exposure to artificial bile is also shown

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