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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Rational engineering of Kluyveromyces marxianus to create a chassis for the production of aromatic products

Fig. 5

Further integration of precursor supply in a phenylalanine overproducing strain. a Beginning with strain KmASR.047 with the non-oxidative PPP overexpressed, we attempted to further increase PEP supply using EcppsA, the PEP synthase from E.coli, a PPDK from A. thaliana and the K. marxianus enolase, with only the last increasing phenyalanine/-2-PE production. b Attempts to further increase E4P supply using Xfpk resulted in net decreases in phenylalanine, tyrosine and shikimate production. Expressing a Pta only allowed aromatic compound production to recover if an enzyme was coexpressed to hypothetically simultaneously increase PEP supply. We also found that the Pta from S. enterica was more effective in this role. Data are the mean ± s.d. of at least three biological replicates. Km: Kluyveromyces marxianus, At: Arabidopsis thaliana, Bb: Bifidobacterium breve, Ct: Chaetomium thermophilum, An: Aspergillus nomius, Hm: Haloferax mediterranei, Bs: Bacillus subtilis, Se: Salmonella enterica

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