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Table 2 List of clones selected from each category of mutants

From: Differential role of segments of α-mating factor secretion signal in Pichia pastoris towards granulocyte colony-stimulating factor emerging from a wild type or codon optimized copy of the gene

Mutations WT-GCSF CO-GCSF
Matα:WT 2 6, 10
Matα:Δ57–70 6, 16 25, 32
Matα:Δ30–43 10, 16 12, 32
Matα:Δ47–49 24, 30 44, 45
Matα:Δ57–70;30–43 21, 34 23, 33
Matα:Δ57–70;47–49 22, 25 17, 18