From: Differential role of segments of α-mating factor secretion signal in Pichia pastoris towards granulocyte colony-stimulating factor emerging from a wild type or codon optimized copy of the gene
Mutations
WT-GCSF
CO-GCSF
Matα:WT
2
6, 10
Matα:Δ57–70
6, 16
25, 32
Matα:Δ30–43
10, 16
12, 32
Matα:Δ47–49
24, 30
44, 45
Matα:Δ57–70;30–43
21, 34
23, 33
Matα:Δ57–70;47–49
22, 25
17, 18