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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Differential role of segments of α-mating factor secretion signal in Pichia pastoris towards granulocyte colony-stimulating factor emerging from a wild type or codon optimized copy of the gene

Fig. 3

a SDS-PAGE analysis of extracellular G-CSF produced in BMMY medium by the truncated Matα fused to the WT-GCSF gene. Equal volume (25 μl) of the culture filtrate was loaded after 72 h of cultivation in a 48-well plate. Representative image of Matα:Δ57–70 transformants is shown. Lanes 1 & 11: Mol wt markers; Lane 2: empty vector; Lanes 3, 4, 5, 6, 8, 9, 10: Cl #s 3 to 9; Lane 12: Matα:WT; Lanes 13, 14, 15, 17, 18, 19, 20: Cl #s 13 to 19; Lanes 7 & 16: Standard Filgrastim (2.0 μg & 2.5 μg respectively)  b Average extracellular G-CSF (mg/L) production from 14/15 clones of each category at microplate level 72 h post-methanol induction

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Microbial Cell Factories

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