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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Differential role of segments of α-mating factor secretion signal in Pichia pastoris towards granulocyte colony-stimulating factor emerging from a wild type or codon optimized copy of the gene

Fig. 2

Scheme for construction of the plasmid containing the CO-GCSF gene. A copy of the CO-GCSF gene was amplified from pPICZαB-HSADIII-CO-GCSF construct [50] using G-CSF specific forward and reverse primers containing appropriate restriction sites. The restriction enzyme digested (Xho I and Xba I) vector and the amplified G-CSF fragment were isolated from the gel, purified and ligated to form the pPICZαB-CO-GCSF 

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