Table 2 Yeast strains used in this study
From: Enhanced production of taxadiene in Saccharomyces cerevisiae
Strain name | Genotype | Reference |
|---|---|---|
GTy116 | MATa, leu2-3, 112::HIS3MX6-GAL1p-ERG19/GAL10p-ERG8; ura3-52::URA3-GAL1p-MvaSA110G/GAL10p-MvaE (codon optimised); his3Δ1::hphMX4-GAL1p-ERG12/GAL10p-IDI1; trp1-289::TRP1_GAL1p-CrtE(X.den)/GAL10p-ERG20; YPRCdelta15::NatMX-GAL1p-CrtE(opt)/GAL10p-CrtE | [9] |
LRS1 | mGTy116 Yep_Gal1p-3Xflag-M60TASYBRE-CYC1 | This study |
LRS2 | mGTy116 ARS1014a::TDH3p-TASY-ADH1t | [9] |
LRS3 | mGTy116 ARS1014a::Gal1p-TASY-GFP | This study |
LRS4 | LRS3 ARS1622:: Gal1p-MBP-TASY-ERG20* | This study |
LRS5 | LRS4 ARS1114a::Gal1p-MBP-TASY-ERG20* | This study |