Skip to main content

Table 2 Yeast strains used in this study

From: Enhanced production of taxadiene in Saccharomyces cerevisiae

Strain name Genotype Reference
GTy116 MATa, leu2-3, 112::HIS3MX6-GAL1p-ERG19/GAL10p-ERG8; ura3-52::URA3-GAL1p-MvaSA110G/GAL10p-MvaE (codon optimised); his3Δ1::hphMX4-GAL1p-ERG12/GAL10p-IDI1; trp1-289::TRP1_GAL1p-CrtE(X.den)/GAL10p-ERG20; YPRCdelta15::NatMX-GAL1p-CrtE(opt)/GAL10p-CrtE [9]
LRS1 mGTy116 Yep_Gal1p-3Xflag-M60TASYBRE-CYC1 This study
LRS2 mGTy116 ARS1014a::TDH3p-TASY-ADH1t [9]
LRS3 mGTy116 ARS1014a::Gal1p-TASY-GFP This study
LRS4 LRS3 ARS1622:: Gal1p-MBP-TASY-ERG20* This study
LRS5 LRS4 ARS1114a::Gal1p-MBP-TASY-ERG20* This study