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Table 1 Summary of previous taxadiene biosynthesis yields and conditions using E. coli and S. cerevisiae as microbial hosts

From: Enhanced production of taxadiene in Saccharomyces cerevisiae

Host Taxadiene concentration Condition Reference
E. coli  ~ 300 mg/L 2 mL rich media, 22° C, IPTG induction, 5 days [7]
E. coli 1.02 ± 0.08 g/L 1 L fed-batch cultivation, 22 °C, IPTG induction [7]
E. coli 94% of total product IPTG-induced taxadiene synthase purification from E. coli (temperature shift from 37 to 20 °C). A cell-free assay was conducted afterwards. [14]
E. coli 1.3 mg/L 1 L Luria–Bertani medium culture, IPTG-induced taxadiene synthase expression (temperature shift from 37 °C to room temperature) [15]
S. cerevisiae 8.7 ± 0.85 mg/L 100 mL buffered YPD medium in 500 mL baffled shake flask, two days, 28 °C [8]
S. cerevisiae 20 mg/L 5 mL YP-galactose medium culture [9]
S. cerevisiae 0.7 mg/L 700 mL rich YP-Galactose medium, 65 h [13]
S. cerevisiae 1.0 mg/L 700 mL Selective SG-URA medium, 65 h [13]