Skip to main content

Table 1 Strains and plasmids used in this study

From: Intelligent microbial cell factory with genetic pH shooting (GPS) for cell self-responsive base/acid regulation

Strains Relevant genotype Reference or source
E. coli BM Top 10 F mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ Purchased from biomed
E. coli BL21(DE3) E. coli str. B F ompT gal dcm lon hsdSB(rBmB) λ(DE3) [lacI lacUV5-T7p07 ind1 sam7 nin5] [malB+]K-12S) Purchased from biomed
E. coli BW-BIE The strains for lycopene biosynthesis [27]
BM-pSB1C3 E. coli BM Top10 carrying the plasmid pSB1C3 This study
BM-pSBASC E. coli BM Top10 carrying the plasmid pSB-ASC This study
BM-pSBBSC E. coli BM Top10 carrying the plasmid pSB-BSC This study
BL-pET28a E. coli BL21(DE3) carrying the plasmid pET28a This study
BL-pETglsA E. coli BL21(DE3) carrying the plasmid pET-glsA This study
BL-pETgadA E. coli BL21(DE3) carrying the plasmid pET-gadA This study
BW-pUCASC E. coli BW-BIE carrying the plasmid pUC-ASC This study
BW-pUCBSC E. coli BW-BIE carrying the plasmid pUC-BSC This study
BW-pUCATB E. coli BW-BIE carrying the plasmid pUC-ATB This study
Plasmids
 pSB1C3 The wildly used backbone for gene cloning in synthetic biology iGEM Registry
 pUC19 Cloning vector, Ampr Purchased from BioMed
 pET28a Expression vector, kanr Lab Preserved
 pSB-ASC pSB1C3 carrying the acid regulation circuit (P-asr + glsA) This study
 pSB-BSC pSB1C3 carrying the base regulation circuit (P-apt2 + ldhA) This study
 pETglsA pET28a inserted by glsA gene in the multiple cloning sites This study
 pETgadA pET28a inserted by gadA gene in the multiple cloning sites This study
 pUCASC pUC19 carrying the acid shooting circuit (P-asr + glsA) This study
 pUCBSC pUC19 carrying the base shooting circuit (P-atp2 + ldhA) This study
 pUCATB pUC19 carrying the genetic pH shooting circuits with a terminator between the two circuits ASC and BSC This study