Table 1 Strains and plasmids used in this study
Strains | Relevant genotype | Reference or source |
|---|---|---|
E. coli BM Top 10 | F– mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ− | Purchased from biomed |
E. coli BL21(DE3) | E. coli str. B F– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3) [lacI lacUV5-T7p07 ind1 sam7 nin5] [malB+]K-12(λS) | Purchased from biomed |
E. coli BW-BIE | The strains for lycopene biosynthesis | [27] |
BM-pSB1C3 | E. coli BM Top10 carrying the plasmid pSB1C3 | This study |
BM-pSBASC | E. coli BM Top10 carrying the plasmid pSB-ASC | This study |
BM-pSBBSC | E. coli BM Top10 carrying the plasmid pSB-BSC | This study |
BL-pET28a | E. coli BL21(DE3) carrying the plasmid pET28a | This study |
BL-pETglsA | E. coli BL21(DE3) carrying the plasmid pET-glsA | This study |
BL-pETgadA | E. coli BL21(DE3) carrying the plasmid pET-gadA | This study |
BW-pUCASC | E. coli BW-BIE carrying the plasmid pUC-ASC | This study |
BW-pUCBSC | E. coli BW-BIE carrying the plasmid pUC-BSC | This study |
BW-pUCATB | E. coli BW-BIE carrying the plasmid pUC-ATB | This study |
Plasmids | ||
pSB1C3 | The wildly used backbone for gene cloning in synthetic biology | iGEM Registry |
pUC19 | Cloning vector, Ampr | Purchased from BioMed |
pET28a | Expression vector, kanr | Lab Preserved |
pSB-ASC | pSB1C3 carrying the acid regulation circuit (P-asr + glsA) | This study |
pSB-BSC | pSB1C3 carrying the base regulation circuit (P-apt2 + ldhA) | This study |
pETglsA | pET28a inserted by glsA gene in the multiple cloning sites | This study |
pETgadA | pET28a inserted by gadA gene in the multiple cloning sites | This study |
pUCASC | pUC19 carrying the acid shooting circuit (P-asr + glsA) | This study |
pUCBSC | pUC19 carrying the base shooting circuit (P-atp2 + ldhA) | This study |
pUCATB | pUC19 carrying the genetic pH shooting circuits with a terminator between the two circuits ASC and BSC | This study |