Skip to main content

Table 2. Genotypes, features and JBEI registry IDs of plasmid constructs and R. toruloides strains investigated in this study

From: Conversion of poplar biomass into high-energy density tricyclic sesquiterpene jet fuel blendstocks

Plasmids

Genotypes/features

Source/references

JBEI registry ID

Construct 1

PGAPDH-ScEIZS-NATR

This study

JPUB_013517

Construct 2

PGAPDH-RtEIZS-NATR

This study

JPUB_013519

Construct 3

PTEF1-RtEIZS-HYGR

This study

JPUB_013521

Construct 4

PANT-RtEIZS-PTEF1-RtEIZS-NATR

This study

JPUB_013523

Construct 5

PGAPDH-LpPPS-NATR

This study

JPUB_013525

Construct 6

PGAPDH-RtPPS-NATR

This study

JPUB_013527

Construct 7

PTEF1-RtPPS-HYGR

This study

JPUB_013529

Construct 8

PANT-RtPPS-PTEF1-RtPPS-NATR

This study

JPUB_013531

Strains

IFO0880 (WT)

Rhodosporidium toruloides strain IFO0880, mating type A2

NBRC culture collection

 

EIZS1

IFO0880/PTEF1-RtEIZS-HYGR

This study

JPUB_013534

EIZS2

IFO0880/PGAPDH-RtEIZS-NATR

This study

JPUB_013532

EIZS3

IFO0880/PGAPDH-RtEIZS-NATR/PTEF1-RtEIZS-HYGR

This study

JPUB_013533

EIZS4

IFO0880/PANT-RtEIZS-PTEF1-RtEIZS-NATR

This study

JPUB_013535

EIZS5

IFO0880/PANT-RtEIZS-PTEF1-RtEIZS-NATR/PTEF1-RtEIZS-HYGR

This study

JPUB_013536

PPS1

IFO0880/PTEF1-RtPPS-HYGR

This study

JPUB_013539

PPS2

IFO0880/PGAPDH-RtPPS-NATR

This study

JPUB_013537

PPS3

IFO0880/PGAPDH-RtPPS-NATR/PTEF1-RtPPS-HYGR

This study

JPUB_013538

PPS4

IFO0880/PANT-RtPPS-PTEF1-RtPPS-NATR

This study

JPUB_013540

PPS5

IFO0880/PANT-RtPPS-PTEF1-RtPPS-NATR/PTEF1-RtPPS-HYGR

This study

JPUB_013541

  1. GAPDH glyceraldehyde 3-phosphate dehydrogenase, TEF1 translational elongation factor, ANT adenine nucleotide translocase, ScEIZS epi-isozizaene synthase from Streptomyces coelicolor A3(2) (NCBI accession number, WP_011030119.1), RtEIZS epi-isozizaene synthase from S. coelicolor A3(2) codon optimized for R. toruloides, LpPPS prespatane synthase from Laurencia pacifica (ASV63464.1), RtPPS prespatane synthase from L. pacifica codon optimized for R. toruloides, NATR nourseothricin resistance; HYGR, hygromycin B resistance