Table 2 Primers used in this study
Name | 5′ → 3′ DNA sequences |
|---|---|
For recombinant construction | |
TU1-F | GATGCTGTTGTTCTTTCTTCTGTTT |
TU1-R | CCTGCAGCGTACGAAGCTTCAGCTGAGTTCTATGTCTTAATAAGTCTGTA |
KAN1-F | TACAGACTTATTAAGACATAGAACTCAGCTGAAGCTTCGTACGCTGCAGG |
KAN1-R | GATCGTCTCATTTGCATCGGGTTCAGCATAGGCCACTAGTGGATCTGATA |
TD1-F | TATCAGATCCACTAGTGGCCTATGCTGAACCCGATGCAAATGAGACGATC |
TD1-R | ACTTTCTAAAATGGCTATATAGGGG |
TU2-R | TTCAGTTTTGGATAGATCAGTTAGAAGTTCTATGTCTTAATAAGTCTGTA |
PGKP-F | TACAGACTTATTAAGACATAGAACTTCTAACTGATCTATCCAAAACTGAA |
PGKP-R | TTTCTGGATGATCAGAAATAGTCATGTTTTATATTTGTTGTAAAAAGTAG |
MAL-F | CTACTTTTTACAACAAATATAAAACATGACTATTTCTGATCATCCAGAAA |
MAL-R | AGAAAAGAAAAAAATTGATCTATCGTTATTTGACGAGGTAGATTCTACCT |
PGKT-F | AGGTAGAATCTACCTCGTCAAATAACGATAGATCAATTTTTTTCTTTTCT |
PGKT-R | CCTGCAGCGTACGAAGCTTCAGCTGTAACGAACGCAGAATTTTCGAGTTA |
KAN2-F | TAACTCGAAAATTCTGCGTTCGTTACAGCTGAAGCTTCGTACGCTGCAGG |
For PCR verification | |
UUK-F | ATCTAAGAGGACGGTTGCTG |
UUK-R | GTCAAGACTGTCAAGGAGGG |
KDD1-F | TCGCAGACCGATACCAGGAT |
KDD1-R | TCAACGGATGGGAAAGCAAT |
UUP-F | GCGGTCCGTTCTGTGGTT |
UUP-R | CCCTCTGTGGCGGTCTAT |
PPM-F | CACATGCTATGATGCCCACT |
PPM-R | CGCAAACAAACGGAGGTA |
MPT-F | CGAAAGATAAGCCCAATG |
MPT-R | CTGTAACGAACGCAGAAT |
PTK-F | AAATTCTGCGTTCGTTAC |
PTK-R | CCGTCAGCCAGTTTAGTC |
KDD2-F | TATGTGAATGCTGGTCGCTAT |
KDD2-R | CCGTTGCTACTGCCGTTA |
For RT-qPCR | |
qGDB1-F | AGCCTAACTTCGGCACTC |
qGDB1-R | CACCGTCATCTAATCTCAAATA |
qEMI2-F | GGCAAGGATGTCGTGAGGTT |
qEMI2-R | AGCCTGAAGTGTAGCAGTGG |
qGLK1-F | ATCACGAAGTTGCCACAG |
qGLK1-R | TCACCCAAGAACATCCCT |
qHXK2-F | TCCGTTTACAACAGATACCC |
qHXK2-R | ATAACAGCGGCACCAGCA |
qHXK1-F | GTGTCAAGACCACTCTGCCA |
qHXK1-R | GGATCTTTGCTTGCGTCACC |
qPGM2-F | GAAAAGGACGGTGTTTGGGC |
qPGM2-R | GGCTGGGAAGGCGGAATTAA |
qPRM15-F | TAAGCAAGACCGCAACCCAA |
qPRM15-R | CCAATCCCTGAGACGCTTGT |
qUGP1-F | CGAGAGCAACACAAACAGCG |
qUGP1-R | CCGGGTTGGGAGACTTGATC |
qTPS1-F | GGGGCAAGGTTGTTCTG |
qTPS1-R | TCACGGGTGGACGAGAC |
qTPS2-F | CCACCACTGCCCAAGACAAT |
qTPS2-R | CAGGTTGCGTTCGGTTCTTG |
qTPS3-F | TGCTCCGTCTGCTAGAGTCT |
qTPS3-R | GGATCGACATCTGGAACGCT |
qUBC6-F [59] | GGACCTGCGGATACTCCTTAC |
qUBC6-R [59] | TAATCGTGTGTTGGGCTTGA |