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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Haemagglutinin displayed on the surface of Lactococcus lactis confers broad cross-clade protection against different H5N1 viruses in chickens

Fig. 1

Characterization of the HA protein displayed on the surface of L. lactis. a Schematic diagram of L. lactis/pNZ8148-Spax-HA. A GS linker was inserted between Spax and HA to stabilize the expression of the HA protein. b Western blot analysis. Lane 1: Precision Plus Protein™ WesternC™ (Bio-Rad, USA) marker; Lane 2: L. lactis/pNZ8148-Spax-HA; Lane 3: L. lactis-pNZ8148-Spax. c Immunofluorescence microscopy assay of the HA protein: L. lactis/pNZ8148-Spax (left) and L. lactis/pNZ8148-Spax-HA (right) (magnification: 1,000 ×). (d) Flow cytometric analysis of the HA protein displayed on the surface of L. lactis: L. lactis/pNZ8148-Spax (left) and L. lactis/pNZ8148-Spax-HA (positive rate: 60.5%) (right). A total of 15,000 cells were counted. (e) Quantification of the HA protein expressed by L. lactis/pNZ8148-Spax-HA by indirect ELISA. The values were determined from three independent experiments, and the bars indicate the means ± SDs

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Microbial Cell Factories

ISSN: 1475-2859

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