Table 1 Consensus design information and experimental characterization results of the single-site mutants

M0

–

–

–

11.6

1

100

L6P^c

Loop

12.2

58.97

19

1.64

94.28 ± 9.54

D17V^c

Loop

11.2

43.04

150

12.9

105.00 ± 5.71

P20T

Loop

11.2

61.00

8.7

0.75

95.24 ± 4.77

V33L

α-Helix

15.6

59.58

5.45

0.47

114.29 ± 11.32

C52N

α-Helix

9.4

91.21

7.85

0.68

110.47 ± 6.67

G58D^c

β-Turn

11.9

61.00

17

1.47

83.80 ± 10.41

W59F

β-Turn

8

85.88

3

0.26

120.95 ± 6.77

D73T

β-Turn

20.8

68.85

8.1

0.70

106.67 ± 17.41

F108Y^c

α-Helix

7.8

71.10

16

1.37

105.00 ± 5.71

Y109F^c

α-Helix

8.3

69.85

13

1.12

122.86 ± 1.91

L162A

β-Turn

13.0

66.42

10.4

0.89

108.57 ± 4.35

T117P^c

β-Turn

21.0

44.84

12

1.03

100.92 ± 15.57

Q165I^c

α-Helix

9.8

60.03

20.7

1.78

88.57 ± 9.62

K166A

α-Helix

10.2

51.51

11.3

0.97

103.81 ± 7.56

T199S^c

α-Helix

10.2

42.81

14

1.2

117.14 ± 8.60

T251C^c

β-Sheet

6.3

78.10

23

1.98

107.62 ± 2.09

E349V^c

Loop

14.3

96.20

12

1.03

114.29 ± 13.29

K351E^c

Loop

15.7

83.23

13.7

1.18

142.86 ± 4.85

V362I

β-Sheet

6.8

48.15

10.9

0.94

99.05 ± 15.23

V340L

Loop

6.2

66.40

3.8

0.33

80.00 ± 15.57

C331S

Loop

6.2

83.72

6

0.52

121.90 ± 1.90