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Table 2 Physiology data in chemostat cultures

From: Engineering cofactor metabolism for improved protein and glucoamylase production in Aspergillus niger

 

B36

OE gsdA

OE gndA

OE maeA

µexponential phase (h−1)

0.21 ± 0.01

0.21 ± 0.02

0.21 ± 0.01

0.19 ± 0.01

Cbiomass (gDCW/kg)

5.66 ± 0.16

7.5 ± 0.36

6.46 ± 0.17

4.74 ± 0.29

qCO2 (mmol/gDCW·h)

1.33 ± 0.04

1.00 ± 0.03

1.1 ± 0.03

1.45 ± 0.09

qO2 (mmol/gDCW·h)

1.6 ± 0.18

1.19 ± 0.15

1.51 ± 0.11

1.79 ± 0.20

RQ

0.83 ± 0.09

0.81 ± 0.1

0.73 ± 0.06

0.81 ± 0.09

qProtein (mg/gDCW·h)

3.71 ± 0.41

2.27 ± 0.37

5.62 ± 1

4.94 ± 0.49

qs (mmolmaltose /gDCW·h)

0.39 ± 0.011

0.31 ± 0.009

0.35 ± 0.031

0.47 ± 0.011

YGlaA/X (U/gDCW)

23.84 ± 1.08

14.38 ± 0.82

38.88 ± 3.6

30.83 ± 0.1

C-recovery

99%

110%

104%

91%

  1. Data for strain B36 and three Tet-on driven overexpression strains derived thereof are shown for chemostat cultures with maltose as growth-limiting substrate. Standard deviations ( ±) are given for mean values of duplicate independent steady state results which were measured in technical triplicates. OE gsdA: strain overexpressing gene gsdA; OE gndA: strain overexpressing gene gndA; OE maeA: strain overexpressing gene maeA. Cbiomass, biomass concentration (dry cell weight (DCW)); qCO2, specific carbon dioxide evolution rate; qO2, specific oxygen uptake rate; RQ, respiratory quotient calculated as the ratio of CO2 production and O2 consumption rates; qprotein, specific production rate of extracellular protein; qs, specific substrate consumption rate; YGlaA/X, yield of total glucoamylase activity on biomass; C-recovery, carbon recovery