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Table 2 Physiology data in chemostat cultures

From: Engineering cofactor metabolism for improved protein and glucoamylase production in Aspergillus niger

  B36 OE gsdA OE gndA OE maeA
µexponential phase (h−1) 0.21 ± 0.01 0.21 ± 0.02 0.21 ± 0.01 0.19 ± 0.01
Cbiomass (gDCW/kg) 5.66 ± 0.16 7.5 ± 0.36 6.46 ± 0.17 4.74 ± 0.29
qCO2 (mmol/gDCW·h) 1.33 ± 0.04 1.00 ± 0.03 1.1 ± 0.03 1.45 ± 0.09
qO2 (mmol/gDCW·h) 1.6 ± 0.18 1.19 ± 0.15 1.51 ± 0.11 1.79 ± 0.20
RQ 0.83 ± 0.09 0.81 ± 0.1 0.73 ± 0.06 0.81 ± 0.09
qProtein (mg/gDCW·h) 3.71 ± 0.41 2.27 ± 0.37 5.62 ± 1 4.94 ± 0.49
qs (mmolmaltose /gDCW·h) 0.39 ± 0.011 0.31 ± 0.009 0.35 ± 0.031 0.47 ± 0.011
YGlaA/X (U/gDCW) 23.84 ± 1.08 14.38 ± 0.82 38.88 ± 3.6 30.83 ± 0.1
C-recovery 99% 110% 104% 91%
  1. Data for strain B36 and three Tet-on driven overexpression strains derived thereof are shown for chemostat cultures with maltose as growth-limiting substrate. Standard deviations ( ±) are given for mean values of duplicate independent steady state results which were measured in technical triplicates. OE gsdA: strain overexpressing gene gsdA; OE gndA: strain overexpressing gene gndA; OE maeA: strain overexpressing gene maeA. Cbiomass, biomass concentration (dry cell weight (DCW)); qCO2, specific carbon dioxide evolution rate; qO2, specific oxygen uptake rate; RQ, respiratory quotient calculated as the ratio of CO2 production and O2 consumption rates; qprotein, specific production rate of extracellular protein; qs, specific substrate consumption rate; YGlaA/X, yield of total glucoamylase activity on biomass; C-recovery, carbon recovery