| |
B36
|
OE gsdA
|
OE gndA
|
OE maeA
|
|---|
|
µexponential phase (h−1)
|
0.21 ± 0.01
|
0.21 ± 0.02
|
0.21 ± 0.01
|
0.19 ± 0.01
|
|
Cbiomass (gDCW/kg)
|
5.66 ± 0.16
|
7.5 ± 0.36
|
6.46 ± 0.17
|
4.74 ± 0.29
|
|
qCO2 (mmol/gDCW·h)
|
1.33 ± 0.04
|
1.00 ± 0.03
|
1.1 ± 0.03
|
1.45 ± 0.09
|
|
qO2 (mmol/gDCW·h)
|
1.6 ± 0.18
|
1.19 ± 0.15
|
1.51 ± 0.11
|
1.79 ± 0.20
|
|
RQ
|
0.83 ± 0.09
|
0.81 ± 0.1
|
0.73 ± 0.06
|
0.81 ± 0.09
|
|
qProtein (mg/gDCW·h)
|
3.71 ± 0.41
|
2.27 ± 0.37
|
5.62 ± 1
|
4.94 ± 0.49
|
|
qs (mmolmaltose /gDCW·h)
|
0.39 ± 0.011
|
0.31 ± 0.009
|
0.35 ± 0.031
|
0.47 ± 0.011
|
|
YGlaA/X (U/gDCW)
|
23.84 ± 1.08
|
14.38 ± 0.82
|
38.88 ± 3.6
|
30.83 ± 0.1
|
|
C-recovery
|
99%
|
110%
|
104%
|
91%
|
- Data for strain B36 and three Tet-on driven overexpression strains derived thereof are shown for chemostat cultures with maltose as growth-limiting substrate. Standard deviations ( ±) are given for mean values of duplicate independent steady state results which were measured in technical triplicates. OE gsdA: strain overexpressing gene gsdA; OE gndA: strain overexpressing gene gndA; OE maeA: strain overexpressing gene maeA. Cbiomass, biomass concentration (dry cell weight (DCW)); qCO2, specific carbon dioxide evolution rate; qO2, specific oxygen uptake rate; RQ, respiratory quotient calculated as the ratio of CO2 production and O2 consumption rates; qprotein, specific production rate of extracellular protein; qs, specific substrate consumption rate; YGlaA/X, yield of total glucoamylase activity on biomass; C-recovery, carbon recovery
