Engineering cofactor metabolism for improved protein and glucoamylase production in Aspergillus niger

Table 1 GSMM-predicted NADPH producing reactions in A. niger

Rn name	Rn description	Formula	Gene
R25	Glucose 6-phosphate-dehydrogenase (gsdA)	G6P + NADP = > D6PGL + NADPH + H	An02g12140
R27	Phosphogluconate dehydrogenase (gndA)	6PGC + NADP = > Ru5P + CO₂ + NADP	An11g02040
R36	Isocitrate dehydrogenase (icdA) (NADP +)	ICIT[m] + NADP[m] = > AKG[m] + CO₂[m] + NADPH[m]	An02g12430
R38	Isocitrate dehydrogenase (NADP +)	ICIT + NADP = > AKG + CO₂ + NADPH	An02g12430
R55	Malic enzyme (NADP-specific) (maeA)	MAL + NADP = > PYR + CO₂ + NADPH	An05g00930
R57	Malic enzyme (NADP-specific)	MAL[m] + NADP [m] = > PYR [m] + CO₂[m] + NADPH [m]	An05g00930
R110	(S)-3-Hydroxybutanoyl-CoA: NADP + oxidoreductase	3HBCoA[m] + NADP [m] < = > AACCoA[m] + NADPH [m] +H[m]	An14g00430
R125	dihydrofolate:NADP + oxidoreductase	NADP [m] + DHF[m] < = > NADPH [m] + FOLATE[m]	An12g04590
R190	Alcohol dehydrogenase	ETH + NADP < = > ACAL + NADPH + H	An16g02510

[m] reactions in the mitochondrion; Rn, reaction. All reactions listed here were predicted in Lu et al. [31]

ISSN: 1475-2859