Fig. 2

Data for shake flask-level cultivations of all engineered strains in the YS20.2 background in relation to the control strain B36. a Dry cell weight (DCW); b Relative expression level of glaA and engineered genes; c Total secreted protein per gram biomass at 72 h after inoculation; d Enzyme activity of GlaA per gram biomass at 72 h after inoculation; e Intracellular NADPH concentration in the exponential phase; f Comparison between engineered strains in the AB4.1 and YS20.2 background, respectively. All experiments were conducted in biological quadruplicates. Significance values were calculated with the two-tailed t-test with independent variables (*p < 0.05, **p < 0.01, ***p < 0.001)