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Table 1 Details of primers used in this study

From: Immunogenicity evaluation of recombinant Lactobacillus casei W56 expressing bovine viral diarrhea virus E2 protein in conjunction with cholera toxin B subunit as an adjuvant

Primers

Sequences (5′–3′)

E2-F

GAGCTCaATGCTCCCAGCCTGTAAACC

E2-R

GGGCCCAAACCGGAATTCACCTAAGGTCGTTTGTTCTGAT

ctxB-F

GAATTCGGTGGTGGTGGTTCTGGTGGTGGTGGTTCTbATGATTAAATTAAAATTTGGTGTTT

ctxB-R

GGGCCCTTAATTTGCCATACTAATTGC

5′UTR-F

GGTAGCAACAGTGGTGAG

5′UTR-R

GTAGCAATACAGTGGGCC

  1. aUnderlining indicates restriction enzyme recognize site
  2. bItalic indicates the gene encoding flexible linker (GGGGS)2
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Microbial Cell Factories

ISSN: 1475-2859

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