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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Immunogenicity evaluation of recombinant Lactobacillus casei W56 expressing bovine viral diarrhea virus E2 protein in conjunction with cholera toxin B subunit as an adjuvant

Fig. 1

Construction of the recombinant Lactobacillus strains and identification of the proteins of interest expressed by the recombinant Lactobacillus strains. a Schematic illustration of the construction of the recombinant pPG-E2 and pPG-E2-ctxB plasmids. b Identification of the proteins of interest expressed by the recombinant strains pPG-E2/Lc W56 and pPG-E2-ctxB/Lc W56 using Western blot with mouse anti-E2 or anti-ctxB mAb, respectively. c Identification of the E2 protein expressed by pPG-E2/Lc W56 by IFA. d Identification of the fusion protein E2-ctxB expressed by pPG-E2-ctxB/Lc W56 by IFA

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