Table 3 Specific PCR used in this study

NHM(PCR1)

NlpC-F-SOE: CAAGGAGGAAAAGACCACATGGTAGATGCAAAGAAAGTATTG

95 °C for 5 min, 95 °C for 30 s, 57 °C for 30 s, and 72 °C for 60 s

NlpC-R: GGAAGATCTCCATGGCTCGAGATGATGATGATGATGGTGTAGTGAAGGACGAACAGC

P_slpA(PCR2)

P_slpA-F: CCGGAATTCAAGCGGTAGGTG

95 °C for 5 min, 95 °C for 30 s, 57 °C for 30 s, and 72 °C for 40 s

P_slpA-R-SOE: CAATACTTTCTTTGCATCTACCATGTGGTCTTTTCCTCCTTG

P_slpA-NHM(SOE-PCR)

P_slpA-F: CCGGAATTCAAGCGGTAGGTG

95 °C for 5 min, 95 °C for 30 s, 56 °C for 30 s, and 72 °C for 100 s

NlpC-R: GGAAGATCTCCATGGCTCGAGATGATGATGATGATGGTGTAGTGAAGGACGAACAGC

aiiK(PCR3)

aiiKF: GGAAGATCTATGTGTCAAAATAAAAAGTTGTAC

95 °C for 5 min, 95 °C for 30 s, 54 °C for 30 s, and 72 °C for 60 s

aiiKR: CGGGGTACCTTATTCGTAATACCCTTCCGTTGA

Sequencing

EcoRI-P_SlpA-F: CCGGGAATTCAAGCGGTAGGTGAAATATTAC

95 °C for 5 min, 95 °C for 30 s, 55.5 °C for 30 s, and 72 °C for 120 s

BamHI-T-R: GGCCGGATCCAGCTTGCGTTTGATTTTC