Approaches of QQ | Enzyme type | Origin | Expression strains | Application and references | |
---|---|---|---|---|---|
Purified QQ enzymes | AidP | AHL lactonase | Planococcus sp. | E. coli BL21 | AidP attenuated the pathogenicity of P. carotovorum in Chinese cabbage [2] |
MomL | AHL lactonase | M. olearia Th120 | E. coli BL21(DE3) | MomL attenuated virulence of P. aeruginosa in a Caenorhabditis elegans infection mode [17] | |
AiiK | AHL lactonase | K. huakuii | E. coli BL21(DE3) | AiiK inhibited biofilm formation and attenuated extracellular proteolytic activity and pyocyanin production of P. aeruginosa PAO1 [18] | |
RmmL | AHL lactonase | R. mobilis YJ3 | E. coli BL21(DE3) | RmmL reduced pyocyanin production of P. aeruginosa PAO1 and extracellular protease activity of V. anguillarum VIB72 [19] | |
AiiAQSI-1 | AHL lactonase | Bacillus sp. QSI-1 | E. coli BL21(DE3) | AiiAQSI-1 inhibited swimming motility, extracellular protease, hemolysin factor, and biofilm formation of A. hydrophila YJ-1 [20] | |
AiiAB546 | AHL lactonase | Bacillus sp. B546 | P. pastoris | AiiAB546 decreased mortality rate and delayed mortality time of fish when co-injection with A. hydrophila in common carp [21] | |
Expressed QQ enzymes in pathogenic bacteria | AiiA | AHL lactonase | Bacillus sp. 240B1 | E. carotovora stain SCG1 | The introduction of aiiA gene in E. carotovora stain SCG1 decreased extracellular pectolytic activities and pathogenicity of E. carotovora [22] |
AiiM | AHL lactonase | M. testaceum StLB037 | P. c. c. NBRC 3830 | The introduction of aiiM gene in P. carotovorum subsp. carotovorum NBRC 3830 attenuated soft rot symptoms on potato slices [23] | |
AttM | AHL lactonase | A. tumefaciens c58 | S. scabies | The introduction of attM gene suppressed pathogenicity of S. scabies towards potato tuber [24] | |
HqiA | AHL lactonase | Metagenomic library from soil | P. c. c. CECT 225T | The introduction of hqiA gene in plant pathogen P. carotovorum efficiently interfered swarming motility and maceration enzymes production [25] | |
Isolated and identified new QQ strains | B. licheniformis T-1 | AHL lactonase | Freshwater culture pond sediment | No | B. licheniformis T-1 reduced pathogenicity of A. hydrophila cb15 in zebrafish coinjection [12] |
Constructed recombinant QQ strains | BbMomL | AHL lactonase | M. olearia Th120 | B. brevis | BbMomL reduced secretion of pathogenic factors and pathogenicity of P. carotovorum subsp. carotovorum and P. aeruginosa PAO1 [26] |
LcAiiK | AHL lactonase | K. huakuii | L. casei MCJΔ1 | LcAiiK attenuated swimming motility, virulence factor production, and biofilm formation of A. hydrophila AH-1 and AH-4 (This study) |