Fig. 5
taken from cell-free lysate and adjusted to the total protein concentration: holo-Cph1 before (solid line) and after incubation with additional PCB (dashed line)
Test expressions of the genes encoding for heme oxygenase, HO1, phycocyanobilin:ferredoxin oxidoreductase, PcyA, and cyanobacterial phytochrome, Cph1 (all under control of the T7 promoter). a Test expressions were performed with three E. coli strains: BL21(DE3), EcN and the newly constructed EcN(T7) strain. Verification of Cph1 (~ 80 kDa) production via His6-tag. Samples were taken before (b) and after (a) induction with 0.5 mM IPTG at 17 °C overnight. Known molecular weights of standard proteins are marked. b Colored pellets of test expressions of different combinations of PCB and holo-Cph1 in different E. coli strains. c Difference spectra of holo-Cph1 produced in different strains. Spectra were