Fig. 2

Test expression of rdmS (T7 promoter) and UV/vis heme spectrum. a SDS-PAGE and Western blot targeting the C-terminal StrepII-tag of rdmS expressed in the E. coli strains BL21(DE3), EcN and the newly constructed EcN(T7). Samples were taken before (b) and after (a) induction with 0.5 mM IPTG. Test expressions were performed at 17 °C overnight. b UV/vis spectrum of 20 µM RdmS produced in EcN(T7) with addition of 10 µM hemin to the growth medium. Shown are the peaks for proteins at 280 nm and the heme Soret band at 408 nm. The inset shows an SDS-PAGE of the elution fractions of the affinity purified RdmS. Known molecular weights of standard proteins are marked