Fig. 7From: The temperature of growth and sporulation modulates the efficiency of spore-display in Bacillus subtilisFluorescence localization of adsorbed-RFP. a Spores carrying the cotC::gfp were produced at 25, 37 or 42 °C, adsorbed with RFP, and observed by fluorescence microscopy. For each condition, the same microscopy fields were observed by phase contrast and fluorescence microscopy (green and red). A blue arrow in each phase-contrast panel (PC) indicates the long axis of the spore used to plot red and green fluorescence intensities. b plots of mean green and red fluorescence intensities along the long axis of 80 different spores. Green and red arrows indicate peaks of GFP and RFP fluorescence intensities, respectively. One pixel corresponds to 1.18 nm. a.u. arbitrary unitBack to article page