Fig. 5

Fluorescence microscopy of spores adsorbed with rhodamine-labeled PPT, LYS or BSA. a Fluorescence of PPT-Rd, LYS-Rd or BSA-Rd upon adsorption to wild type spores grown at 25, 37 or 42 °C. The same microscopy field was observed by phase contrast (PC) and fluorescence microscopy (Rhod). The exposure time was the same for all samples. b Quantitative analysis of the fluorescence of 300 spores as in panel a, performed with the ImageJ software, as previously reported [22]. The Y-axis describes the Total corrected cellular fluorescence (TCCF) value