Fig. 3

Fluorescence microscopy analysis of spores displaying CotG-RFP or CotC-GFP. a–c Spores of strain RH296, carrying the cotG::rfp fusion (a) and of strain RH238, carrying the cotC::gfp fusion (b) were produced at 25, 37 or 42 °C and observed with a fluorescent microscope. The exposure time was the same for all samples with the same reporter gene. Phase contrast (PC), red-fluorescence (CotG-RFP) or green-fluorescence (CotC-GFP) microscopy images of representative spores are reported. Scale bar 1 μm. b–d Quantitative analysis of the fluorescence of more than 300 spores as in panels a–c, performed with the ImageJ software, as previously reported [22]. The Y-axis describes the Total corrected cellular fluorescence (TCCF) value