Fig. 2
Flow cytometry analysis of spores of strains carrying the cotB::tetC or cotC::tetC gene fusion. Spores were produced at 25, 37 or 42 °C and reacted with anti-TTFC antibody, then with FITC-conjugated secondary antibody. The analysis was then performed on the entire spore population (ungated). In each panel is indicated the percentage of positive events above the fluorescent intensity threshold of 1 × 103 (red line). As control experiment spores of an isogenic wild type strain (PY79) not containing any gene fusion were analyzed