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Fig. 2 | Microbial Cell Factories

Fig. 2

From: MsrR is a thiol-based oxidation-sensing regulator of the XRE family that modulates C. glutamicum oxidative stress resistance

Fig. 2

Negative regulation of msrR-3-mst and mfs expressions by MsrR. a, b β-galactosidase analysis of the msrR promoter activity was performed using the transcriptional PmsrR::lacZY chromosomal fusion reporter expressed in indicated strains exposed to H2O2 and CdCl2. c–f qRT-PCR assay was performed to analyze the expression of 3-mst and mfs in indicated strains exposed to H2O2 and CdCl2. The mRNA levels were presented relative to the value obtained from WT cells without treatment. Relative transcript levels of WT strains without stress treatment were set at a value of 1.0. Data show the averages of three independent experiments, and error bars indicated the SDs from three independent experiments. ***P ≤ 0.001; **P ≤ 0.01; *P ≤ 0.05

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