Fig. 1

MsrR was required for optimal growth under various stress. a Physical map of the msrR-3-mst-ncgl2677 genetic cluster and mfs gene in Corynebacterium glutamicum (upper panel) and construction of plasmids for gene disruption (pK18mobsacB derivatives) and complementation (pXMJ19 derivatives) (lower panel). Open reading frames (ORFs) were marked by open arrows, and the deleted regions were in gray. The restriction sites were indicated. msrR, 3-mst, and mfs represented ncgl2679, ncgl2678, and ncgl2680 genes, encoding multiple stress resistance regulator, 3-Mercaptopyruvate sulfurtransferase, and a major facilitator superfamily protein, respectively. b Growth of the indicated three strains in LB broth without stress was used as control. c–j Growth of indicated strains in LB broth with 0.3 mM cumene hydroperoxide (CHP), 0.9 mM menadione (MEN), 45 mM hydrogen peroxide (H₂O₂), 0.4 mM hypochlorous acid (HOCl), 1.5 mM tert-butyl hydroperoxide (t-BHP), 5 mM iodoacetamide (IAM), 0.1 µg/ml gentamicin, or 17 µM cadmium chloride (CdCl₂), respectively. Data show the averages of three independent experiments, and error bars indicated the SDs from three independent experiments. ***P ≤ 0.001; **P ≤ 0.01; *P ≤ 0.05