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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Catabolism of l-rhamnose in A. nidulans proceeds via the non-phosphorylated pathway and is glucose repressed by a CreA-independent mechanism

Fig. 4

Ectopic expression of lraA in the ΔlraA mutant background restores both growth on l-rhamnose and α-l-rhamnosidase activity. Transformation-mediated ectopic replacement of pyroA4 by functional lraA in AR247 (∆lraA). a Plate growth tests of the isogenic lraA+ nutritional control AR271, ∆lraA strains (AR247 and AR248) and four lraA+ complemented transformants (C1-C4) on l-rhamnose after 3 days at 37 °C. b MUR-mediated detection (under UV illumination) of α-l-rhamnosidase activity halos produced by lraA-complemented transformants (C1–C4) and control AR271 (lraA+) strains compared to ∆lraA (AR247 and AR248) after 40 h at RT. c Diagnostic PCRs confirm the absence of the lraA gene (AN4186) on chromosome II (replaced by Af_riboB) and replacement of the mutant pyroA4 allele at locus AN7725 by the lraA expression cassette and Af_pyroA—i.e. substitution of the 2.6 kb DNA fragment in AR247 by the 5.8 kb complementation cassette in transformants C1–C4

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