Fig. 2From: Catabolism of l-rhamnose in A. nidulans proceeds via the non-phosphorylated pathway and is glucose repressed by a CreA-independent mechanismIdentification, gene replacement analysis and growth tests of A. nidulans ΔlraA mutant strains. a Schematic diagram of the lraA/AN4186 locus in AR198 and the gene replacement event in ΔlraA strains. Correct replacement of lraA/AN4186 with the Af_riboB expression cassette in four selected transformants (T5, T6, T9 and T10) was verified by the absence or appearance of PCR products of the expected sizes using CDS-located primers (60, 75 and 195) along with primers 72 and 73 located outside the lraA flanking sequences used in the gene replacement cassette. b A. nidulans ΔlraA mutant (AR247 and AR248) and untransformed (AR198) strains grown (3 days at 37 °C) on solid MM containing different carbon sources (1%) and supplemented with pyridoxine and riboflavin. Equal numbers of conidia (104) were spotted (2 µl) from a sterile suspension in 0.005% Tween 80. Complete genotypes are given in Table 1Back to article page