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Fig. 9 | Microbial Cell Factories

Fig. 9

From: Whole-genome and enzymatic analyses of an androstenedione-producing Mycobacterium strain with residual phytosterol-degrading pathways

Fig. 9

Assay of the substrate conversion catalyzed by KstD211. a Enzyme activity of KstD211 as a function of pH value. b Comparison of the enzyme activity of KstD211 with that of its homologues. NwIB01, ZADF and ZADF4 represent KstD enzymes from Mycobacterium NwIB01, Mycobacterium ZADF and Mycobacterium ZADF4, respectively. c TLC assay of the conversion of 4-AD to ADD catalyzed by KstD211 within 19 h. d HPLC analysis of KstD211-catalyzed turnover of 4-AD to ADD after 48 h. Solid and dashed lines represent HPLC profiles of reaction mixtures at the beginning and the end of reaction, respectively, for 48 h. The conversion rate was calculated based on regression using the area under the curves for two peaks. Error bars indicate standard errors of the means of three repeat experiments

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Microbial Cell Factories

ISSN: 1475-2859

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