Fig. 7From: Whole-genome and enzymatic analyses of an androstenedione-producing Mycobacterium strain with residual phytosterol-degrading pathwaysAssay of the substrate conversion catalyzed by Mon164 and Mon197. a, b SDS-PAGE assay of purified Mon164 and Mon197. M, molecular weight marker. All molecular weight markers were run on the same gel. c, d Turnover of 4-cholesten-3-one by Mon164 and Mon197, respectively, as analyzed by HPLC. Squares, 4-cholesten-3-one; circles, 26-hydro-4-cholesten-3-one. Error bars indicate standard errors of the means of three repeat experimentsBack to article page