Fig. 4

Preparation of soluble cholesterol oxidases. a Group 1 enzymes catalyze an oxidization reaction for the conversion of phytosterols to 4-cholesten-3-one. b–d Genes encoding ChoM1, ChoM2 and Hsd were amplified from the HGMS2 genome by PCR. Arrows indicate expected PCR bands. e–g The ChoM1 (e), ChoM2 (f) and Hsd (g) enzymes were overexpressed in soluble form and fused with His6-MBP. S, supernatant; P, pellet; C, total protein of E. coli BL21(DE3) cells as a control; M, molecular weight marker