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Fig. 10 | Microbial Cell Factories

Fig. 10

From: Whole-genome and enzymatic analyses of an androstenedione-producing Mycobacterium strain with residual phytosterol-degrading pathways

Fig. 10

Preparation of soluble KshA226, KshA395 and KshB122. a Group 5 enzymes can use either 4AD or ADD as substrates to form 9OH-AD or 9OH-ADD, respectively, while 9OH-ADD can simultaneously be converted to HSA through a 9,10-secoreaction. b, c. Agarose gel images of products of the KshA226 and KshA395 genes amplified from the HGMS2 genome by PCR, respectively, indicating the expected sizes. M, molecular weight marker; arrows indicate expected PCR products. d Agarose gel image of the KshB122 gene amplified from the HGMS2 genome by PCR, indicating the expected size. M, DNA marker, which was run on the same gel; lanes 1–6, different PCRs with a gradient of annealing temperatures from 54 to 66 °C with an interval of 2 °C. eg Overexpression of KshA226, KshA395 and KshB122 was evaluated by SDS-PAGE

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