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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Multiple strategies to improve the yield of chitinase a from Bacillus licheniformis in Pichia pastoris to obtain plant growth enhancer and GlcNAc

Fig. 3

The high-cell-density fermentation of the recombinant P. pastoris A4C co-expressing HAC1 in a 5 L fermenter. a The growth curve (OD600), WCW and chitinase activity of fermentation supernatant with different induction time. b SDS-PAGE analysis of fermentation supernatant (8 μL) with different induction time. lane M, Prestained protein marker; lanes 1–12, The fermentation supernatants after being induced for 0, 12, 24, 36, 48, 60, 72, 84, 96, 108, 120 and 132 h, respectively

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Microbial Cell Factories

ISSN: 1475-2859

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