Fig. 8
FSEC profiles of detergent solubilized PfKch11−1094-GFP and GFP-PfKch2fl. GFP-PfKch2fl in FOS-12 (a), LDAO (b), C12E8 (C) and PfKch11−1094-GFP in FOS-12 (d) at 3:1 detergent to protein ratios without any supplement (blue curve), supplemented with cholesteryl-hemisuccinate (green curve), with KCl (black curve) or both (red curve). Elution of solubilized membrane proteins separated on a Superose 6 10/300 GL column were compared with those of molecular weight markers (GE Healthcare Life Science) separated in the same column to determine their approximate size. The Elution of markers were: Blue dextran 2000, 2000 kDa at void volume 8 ml (red asterisk), thyroglobulin 669 kDa at 12.5 ml, ferritin 440 kDa at 14.5 ml, aldolase 158 kDa at 16.3 ml, conalbumin 75 kDa at 17.3 ml, ovalbumin 44 kDa at 17.6 ml. The green asterisk indicates the elution volume of GFP