Fig. 7
Detergent screen for solubilization of GFP tagged PfKch11−1094 and PfKch2fl from crude membranes. Membrane proteins PfKch11−1094-GFP (a) and GFP-PfKch2fl (b) were solubilized using the indicated detergent/protein ratios and a cholesteryl-hemisuccinate concentration of 0.68, 1.36 or 2 mg/ml for the 3 ratios, respectively. Abbreviations; FOS-12, Fos-Choline-12; LDAO lauryldimethylamine N-oxide; Cymal5, 5-Cyclohexyl-1-pentyl-β-d-maltoside; DDM, n-Dodecyl-β-d-maltopyranoside; DM, n-Decyl-β-d-maltopyranoside; C12E8, Octaethylene glycol monododecyl ether; CHAPS, 3-[(3-cholamidopropyl) dimethylam-monio] -1-propanesulfonate; OG, n-Octyl-β-d-glucopyranoside. Solubilization efficiency was defined as GFP fluorescence of solubilized protein normalized to GFP fluorescence in the crude membranes used for solubilization