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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Purification and initial characterization of Plasmodium falciparum K+ channels, PfKch1 and PfKch2 produced in Saccharomyces cerevisiae

Fig. 6

Time and temperature dependent accumulation of PfKch11−1094-GFP and GFP-PfKch2fl. Yeast cell cultures were grown at room temperature (20-25 °C) and at OD450 = 1.0, they were separated in two. One was subsequently incubated at 15 °C and the other at 30 °C. After thermo-equilibration, production of GFP tagged channels was induced by addition of galactose (t = 0). Crude membranes were prepared from cells harvested at the indicated time points. GFP Fluorescence was measured in crude membranes, and translated into pmol channel protein/mg total membrane protein using a GFP standard curve. 15 °C (blue curve) or 30 °C (red curve). a: PfKch11−1094-GFP. b: GFP-PfKch2fl

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