Skip to main content
Fig. 5 | Microbial Cell Factories

Fig. 5

From: CRISPR interference-guided modulation of glucose pathways to boost aconitic acid production in Escherichia coli

Fig. 5

Simultaneous regulation of IDH and PK of E. coli strains in shake-flask cultivation. a Growth curve and glucose consumption of wild-type E. coli BL21(DE3) and recombinant E. coli harboring CRISPRi system. b HPLC analysis of mixed standard and the final fermentation broth of E. coli strains. c Time course of aconitic acid production and its final concentration in E. coli strains. d Relative changes of metabolites in strain E. coli BL21(DE3) + pdCas9-pykF1icdA1 (CCRISPRi) versus control strain (Ccontrol). IDH: isocitrate dehydrogenase (EC 1.1.1.42), encoded by icdA gene; PK: pyruvate kinase (EC 2.7.1.40), encoded by pykA and pykF. WT: wild-type E. coli BL21(DE3) without CRISPRi system; control: recombinant E. coli with non-targeting CRISPRi system; pykF1 + icdA1: recombinant E. coli carrying CRISPRi system simultaneously targeting pykF and icdA genes. Mean ± S.E. (n = 3). **P < 0.01

Back to article page