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Fig. 1 | Microbial Cell Factories

Fig. 1

From: CRISPR interference-guided modulation of glucose pathways to boost aconitic acid production in Escherichia coli

Fig. 1

Schematic diagram of improving aconitic acid production by engineering CRISPRi system in E. coli. Red dashed cross denotes the target enzymes of CRISPRi system. PTS: phosphotransferase system; EMP: Embden-Meyerhof-Parnas pathway; HMP: hexose monophosphate pathway; ED: Entner–Doudoroff pathway; GAC: glyoxylate cycle; TCA: tricarboxylic acid cycle; G-6-P: glucose-6-phosphate; F-6-P: fructose-6-phosphate; F-1,6-P: fructose-1,6-phosphate; 6-P-G: 6-phosphate-gluconolactone; Ru-5-P: ribulose-5-phosphate; R-5-P: ribose-5-phosphate; X-5-P: xylulose-5-phosphate; E-4-P: erythrose-4-phosphate; S-7-P: sedoheptulose-7-phosphate; KDPG: 2-keto-3-deoxy-6-phosphogluconate; GAP: glyceraldehyde-3-phosphate; DHAP: dihydroxyacetone phosphate; PEP: phosphoenolpyruvate; PYR: pyruvate; OAA: oxaloacetate; glk: glucokinase coding gene; pgi: glucose-6-phosphate isomerase coding gene; pfkA and pfkB: 6-phosphofructokinase coding genes; fbaA and fbaB: fructose-bisphosphate aldolase coding genes; tpiA: triose-phosphate isomerase coding gene; zwf: glucose-6-phosphate dehydrogenase coding gene; pgl: 6-phosphogluconolactonase coding gene; gnd: 6-phosphogluconate dehydrogenase coding gene; rpe: ribulose-phosphate 3-epimerase coding gene; rpiA and rpiB: ribose-5-phosphate isomerase coding genes; tktA and tktB: transketolase coding genes; tal: transaldolase coding gene; edd: 6-phosphogluconate dehydratase coding gene; eda: KDPG aldolase coding gene; pckA: phosphoenolpyruvate carboxykinase coding gene; ppc: phosphoenolpyruvate carboxylase coding gene; pykA and pykF: pyruvate kinase coding genes; ldhA: D-lactate dehydrogenase coding gene; pflB: pyruvate formate-lyase coding gene; poxB: pyruvate oxidase coding gene; pta: phosphate acetyltransferase coding gene; ackA: acetate kinase coding gene; acs: acetyl-CoA synthetase coding gene; fdhF: formate dehydrogenase coding gene; adhE: aldehyde-alcohol dehydrogenase coding gene; aceEF: pyruvate dehydrogenase-complex coding genes; maeA and maeB: pyruvic-malic carboxylase coding genes; gltA: citrate synthase coding gene; acnA and acnB: aconitate hydratase coding genes; icdA: isocitrate dehydrogenase coding gene; sucAB: oxoglutarate dehydrogenase coding genes; sucCD: succinyl-CoA synthetase coding genes; sdhABCD: succinate dehydrogenase coding genes; fumA, fumB, fumC and fumD: fumarate hydratase coding genes; mdh: malate dehydrogenase coding gene; aceA: isocitrate lyase coding gene; aceB: malate synthase coding gene

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