Fig. 1From: CRISPR interference-guided modulation of glucose pathways to boost aconitic acid production in Escherichia coliSchematic diagram of improving aconitic acid production by engineering CRISPRi system in E. coli. Red dashed cross denotes the target enzymes of CRISPRi system. PTS: phosphotransferase system; EMP: Embden-Meyerhof-Parnas pathway; HMP: hexose monophosphate pathway; ED: Entner–Doudoroff pathway; GAC: glyoxylate cycle; TCA: tricarboxylic acid cycle; G-6-P: glucose-6-phosphate; F-6-P: fructose-6-phosphate; F-1,6-P: fructose-1,6-phosphate; 6-P-G: 6-phosphate-gluconolactone; Ru-5-P: ribulose-5-phosphate; R-5-P: ribose-5-phosphate; X-5-P: xylulose-5-phosphate; E-4-P: erythrose-4-phosphate; S-7-P: sedoheptulose-7-phosphate; KDPG: 2-keto-3-deoxy-6-phosphogluconate; GAP: glyceraldehyde-3-phosphate; DHAP: dihydroxyacetone phosphate; PEP: phosphoenolpyruvate; PYR: pyruvate; OAA: oxaloacetate; glk: glucokinase coding gene; pgi: glucose-6-phosphate isomerase coding gene; pfkA and pfkB: 6-phosphofructokinase coding genes; fbaA and fbaB: fructose-bisphosphate aldolase coding genes; tpiA: triose-phosphate isomerase coding gene; zwf: glucose-6-phosphate dehydrogenase coding gene; pgl: 6-phosphogluconolactonase coding gene; gnd: 6-phosphogluconate dehydrogenase coding gene; rpe: ribulose-phosphate 3-epimerase coding gene; rpiA and rpiB: ribose-5-phosphate isomerase coding genes; tktA and tktB: transketolase coding genes; tal: transaldolase coding gene; edd: 6-phosphogluconate dehydratase coding gene; eda: KDPG aldolase coding gene; pckA: phosphoenolpyruvate carboxykinase coding gene; ppc: phosphoenolpyruvate carboxylase coding gene; pykA and pykF: pyruvate kinase coding genes; ldhA: D-lactate dehydrogenase coding gene; pflB: pyruvate formate-lyase coding gene; poxB: pyruvate oxidase coding gene; pta: phosphate acetyltransferase coding gene; ackA: acetate kinase coding gene; acs: acetyl-CoA synthetase coding gene; fdhF: formate dehydrogenase coding gene; adhE: aldehyde-alcohol dehydrogenase coding gene; aceEF: pyruvate dehydrogenase-complex coding genes; maeA and maeB: pyruvic-malic carboxylase coding genes; gltA: citrate synthase coding gene; acnA and acnB: aconitate hydratase coding genes; icdA: isocitrate dehydrogenase coding gene; sucAB: oxoglutarate dehydrogenase coding genes; sucCD: succinyl-CoA synthetase coding genes; sdhABCD: succinate dehydrogenase coding genes; fumA, fumB, fumC and fumD: fumarate hydratase coding genes; mdh: malate dehydrogenase coding gene; aceA: isocitrate lyase coding gene; aceB: malate synthase coding geneBack to article page