Table 1 LC-HRMS analysis of SPE-treated supernatants of bacterial cultures expressing a CDPS/CDO couple originating from the same operon

Nocardiopsis potens

cFA

C₁₂H₁₅N₂0⁺₂

219.1128

219.1127

32.75

8.33E+05

∆cFA

C₁₂H₁₃N₂0⁺₂

217.0972

217.0971

33.57

3.49E+04

217.0971

38.58

3.04E+05

∆∆cFA

C₁₂H₁₁N₂0⁺₇

215.0815

–

–

–

cFY

C₁₈H₁₉N₂0⁺₃

311.1390

311.1390

37.41

4.43E+05

∆cFY

C₁₈H₁₇N₂0⁺₃

309.1234

309.1234

38.75

3.34E+05

309.1234

39.57

4.58E+05

∆∆cFY

C₁₈H₁₅N₂0⁺₃

307.1077

307.1077

44.02

6.13E+04

cFM

C₁₄H₁₉N₂0₂S⁺

279.1162

279.1162

39.11

3.92E+06

∆cFM

C₁₄H₁₇N₂0₂S⁺

277.1005

277.1006

40.08

7.76E+05

277.1006

42.16

7.75E+05

∆∆cFM

C₁₄H₁₅N₂0₂S⁺

275.0849

275.0849

43.17

3.86E+04

275.0849

44.05

1.59E+05

cFL

C₁₅H₂₁N₂0⁺₂

261.1598

261.1598

40.93

1.36E+05

∆cFL

C₁₅H₁₉N₂0⁺₂

259.1441

259.1441

41.36

2.17E+05

259.1441

44.26

5.70E+05

∆∆cFL

C₁₅H₁₇N₂0⁺₂

257.1285

257.1284

44.79

1.59E+04

257.1284

45.75

9.23E+04

cFF

C₁₈H₁₉N₂0⁺₂

295.1441

295.1441

41.81

6.13E+06

∆cFF

C₁₈H₁₇N₂0⁺₂

293.1285

293.1285

42.58

4.09E+05

293.1284

43.17

7.77E+06

∆∆cFF

C₁₈H₁₅N₂0⁺₂

291.1128

291.1128

46.98

1.62E+05

291.1128

47.9

2.55E+05

Actinomadura oligospora (pathway 1)

cFY

C₁₈H₁₉N₂0⁺₃

311.1390

311.1390

37.62

1.01E+07

∆cFY

C₁₈H₁₇N₂0⁺₃

309.1234

309.1233

37.17

1.37E+05

309.1234

38.67

4.09E+06

309.1233

39.78

2.26E+06

309.1233

40.14

2.00E+05

∆∆cFY

C₁₈H₁₅N₂0⁺₃

307.1077

307.1077

43.6

8.98E+04

307.1078

44.49

4.63E+05

cYY

C₁₈H₁₉N₂0⁺₄

327.1339

327.1339

29.92

1.25E+05

∆cYY

C₁₈H₁₇N₂0⁺₄

325.1183

325.1183

32.60

2.21E+05

∆∆cYY

C₁₈H₁₅N₂0⁺₄

323.1026

323.1028

41.48

5.52E+04

cWY

C₂₀H₂₀N₃0⁺₃

350.1499

350.1499

37.65

4.37E+05

∆cWY

C₂₀H₁₈N₃0⁺₃

348.1343

–

–

–

∆∆cWY

C₂₀H₁₆N₃0⁺₃

346.1186

–

–

–

Streptomyces catenulae

cFY

C₁₈H₁₉N₂0⁺₃

311.1390

311.1390

37.51

9.80E+06

∆cFY

C₁₈H₁₇N₂0⁺₃

309.1234

309.1233

37.18

9.64E+04

309.1234

38.76

1.89E+06

309.1234

39.59

3.13E+06

309.1234

39.97

1.81E+05

∆∆cFY

C₁₈H₁₅N₂0⁺₃

307.1077

307.1077

42.82

1.74E+04

307.1076

43.64

5.59E+04

cWY

C₂₀H₂₀N₃0⁺₃

350.1499

350.1499

37.66

3.41E+05

∆cWY

C₂₀H₁₈N₃0⁺₃

348.1343

–

–

–

∆∆cWY

C₂₀H₁₆N₃0⁺₃

346.1186

–

–

–

Streptomyces rimosus

cWY

C₂₀H₂₀N₃0⁺₃

350.1499

350.1498

37.40

1.01E+07

∆cWY

C₂₀H₁₈N₃0⁺₃

348.1343

348.1343

38.41

1.81E+06

348.1340

38.73

9.73E+06

348.1342

39.02

2.45E+06

∆∆cWY^b

C₂₀H₁₆N₃0⁺₃

346.1186

–

–

–

cWW

C₂₂H₂₁N₄0⁺₂

373.1659

373.1659

41.62

2.71E+06

∆cWW^b

C₂₂H₁₉N₄0⁺₂

371.1503

–

–

–

∆∆cWW

C₂₂H₁₇N₄0⁺₂

369.1346

–

–

–

Streptomyces sp. NRRL F–5123

cWP

C₁₆H₁₈N₃0⁺₂

284.1394

284.1393

38.08

1.80E+05

∆cWP

C₁₆H₁₆N₃0⁺₂

282.1237

282.1235

37.33

1.80E+07

∆cWP

C₁₆H₁₄N₃0⁺₂

280.1081

280.1081

37.36

1.04E+05

cWL

C₁₇H₂₂N₃0⁺₂

300.1707

300.1706

39.8

1.19E+06

∆cWL

C₁₇H₂₀N₃0⁺₂

298.1550

298.1550

40.64

7.38E+05

∆∆cWL

C₁₇H₁₈N₃0⁺₂

296.1394

–

–

–

Actinomadura oligospora (pathway 2)

cWW

C₂₂H₂₁N₄0⁺₂

373.1659

373.1658

41.04

1.28E+07

∆cWW

C₂₂H₁₉N₄0⁺₂

371.1503

371.1504

41.18

1.50E+05

∆∆cWW

C₂₂H₁₇N₄0⁺₂

369.1346

369.1345

41.15

1.00E+04

Streptomyces aidingensis

cWL

C₁₇H₂₂N₃0⁺₂

300.1707

300.1706

40.06

3.26E+06

∆cWL

C₁₇H₂₀N₃0⁺₂

298.1550

298.1550

40.95

1.28E+07

∆∆cWL

C₁₇H₁₈N₃0⁺₂

296.1394

–

–

–

cFL

C₁₅H₂₁N₂0⁺₂

261.1598

261.1598

41.18

7.00E+04

∆cFL

C₁₅H₁₉N₂0⁺₂

259.1441

–

–

–

∆∆cFL

C₁₅H₁₇N₂0⁺₂

257.1285

–

–

–

cWF

C₂₀H₂₀N₃0⁺₂

334.1550

334.1550

41.65

1.29E+05

∆cWF

C₂₀H₁₈N₃0⁺₂

332.1394

332.1393

42.66

1.96E+05

∆∆cWL

C₂₀H₁₆N₃0⁺₂

330.1237

–

–

–