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Fig. 4 | Microbial Cell Factories

Fig. 4

From: In vivo characterization of the activities of novel cyclodipeptide oxidases: new tools for increasing chemical diversity of bioproduced 2,5-diketopiperazines in Escherichia coli

Fig. 4

Efficiency of CDOs for in vivo dehydrogenation of CDPs. One CDPS and one CDO were co-expressed in E. coli and dehydrogenated CDPs were detected in culture supernatants by LC-MS/MS. CDPSs are indicated on the left by the suffix used to design them in Additional file 1: Table S2. The CDPs they synthesize are also indicated with percentages representing the proportion of each CDP (ratio of UV peak areas) as observed in an experiment without CDO expression (Additional file 1: Table S9). Δ and ΔΔ designate compounds with a [M + H]+ parent ion with a m/z value corresponding to di-dehydrogenated and tetra-dehydrogenated CDP, respectively. Co-expressed CDOs are indicated at the top on the right. The level of detection of each compound is indicated according to data in Additional file 1: Table S5 by a colour and pattern style (top left). When several compounds were detected for one m/z value, their number is indicated, and data reported in this figure correspond to those of the most highly produced compound. The best conditions for producing derivatives of the major CDPs produced by CDPSs are indicated by red rectangles

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